Autophagy Mediates MMP-2 Expression in Glaucomatous Trabecular Meshwork Cells.

ABSTRACT:

Purpose

To investigate the effect of 3-methyladenine (3-MA) and starvation on the expression of matrix metalloproteinase (MMP-2) in patients with primary open-angle glaucoma.

Methods

Primary TM cells were cultured and divided into three groups. The control group was treated with a normal medium, the 3-MA group was stimulated with 3-MA, and the starvation group received nutrient depletion by replacing the normal media with Earle's balanced salt solution. Cellular mRNA and protein were measured at different 3-MA concentrations and starvation time periods. The level of autophagy was accessed by monodansylcadaverine fluorescent staining and expression of specific autophagy-related genes, light chain 3 (LC3), and Beclin1. The effects of 3-MA and starvation on cell proliferation w

SUBMITTER: Xiao YL 

PROVIDER: S-EPMC9536984 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature