Bioinformatics detection of modulators controlling splicing factor-dependent intron retention in the human brain.

ABSTRACT: Alternative RNA splicing is an important means of genetic control and transcriptome diversity. However, when alternative splicing events are studied independently, coordinated splicing modulated by common factors is often not recognized. As a result, the molecular mechanisms of how splicing regulators promote or repress splice site recognition in a context-dependent manner are not well understood. The functional coupling between multiple gene regulatory layers suggests that splicing is modulated by additional genetic or epigenetic components. Here, we developed a bioinformatics approach to identify causal modulators of splicing activity based on the variation of gene expression in large RNA sequencing datasets. We applied this approach in a neurological context with hundreds of dorsolatera

SUBMITTER: Chen SX 

PROVIDER: S-EPMC9537345 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature