Modeling human telencephalic development and autism-associated SHANK3 deficiency using organoids generated from single neural rosettes.

ABSTRACT: Human telencephalon is an evolutionarily advanced brain structure associated with many uniquely human behaviors and disorders. However, cell lineages and molecular pathways implicated in human telencephalic development remain largely unknown. We produce human telencephalic organoids from stem cell-derived single neural rosettes and investigate telencephalic development under normal and pathological conditions. We show that single neural rosette-derived organoids contain pallial and subpallial neural progenitors, excitatory and inhibitory neurons, as well as macroglial and periendothelial cells, and exhibit predictable organization and cytoarchitecture. We comprehensively characterize the properties of neurons in SNR-derived organoids and identify transcriptional programs associated with th

SUBMITTER: Wang Y 

PROVIDER: S-EPMC9537523 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature