Fe₃O₄ Magnetic Nanoparticles Under Static Magnetic Field Improve Osteogenesis via RUNX-2 and Inhibit Osteoclastogenesis by the Induction of Apoptosis.

ABSTRACT:

Purpose

The presented study aimed to investigate the effects of Fe₃O₄ nanoparticles and static magnetic field on osteoblast and osteoclasts' metabolic activity.

Methods

Magnetic nanoparticles were prepared by a wet chemical co-precipitation process and analyzed using X-ray powder diffraction, high-resolution transmission electron microscope (HRTEM), dynamic light scattering (DLS), laser Doppler velocimetry, Raman and the Mössbauer spectroscopy. In vitro experiments were performed using MC3T3, 4B12 and RAW 264.7 cell lines. Cells were cultured in the presence of nanoparticles and with or without exposure to the magnetic field. Proteins were investigated with Western blotting and immunofluorescence and Western blot. Gene expression was analyzed with a qua

SUBMITTER: Marycz K 

PROVIDER: S-EPMC9537728 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature