Chemiluminescent Imaging Assay of SARS-CoV-2 Protein with Target-Induced Enzyme Activity Regulation.

ABSTRACT: Simple but robust testing assays are essential for screening and diagnosis of individuals infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in COVID-19 pandemic. Here, we described a chemiluminescent imaging assay (CLIA) for sensitive and convenient detection of SARS-CoV-2 nucleocapsid protein (NP) by a target-induced enzyme activity regulation (T-EAR) strategy. The T-EAR used a pair of antibody-DNA probes to recognize SARS-CoV-2 NP and proximity-induce rolling circle amplification for mass-production of pyrophosphate to coordinate with Cu²⁺ , which prevented the reduction of Cu²⁺ to Cu⁺ by sodium ascorbate as well as the Cu⁺ -caused inactivation of horseradish peroxidase (HRP). The activity retention of HRP produced stro

SUBMITTER: Chen Y 

PROVIDER: S-EPMC9538728 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature