Scribble basal polarity acquisition in RPE cells and its mislocalization in a pathological AMD-like model.

ABSTRACT: Apicobasal polarity is a hallmark of retinal pigment epithelium cells and is required to perform their functions; however, the precise roles of the different proteins that execute polarity are still poorly understood. Here, we have studied the expression and location of Scribble, the core member of the polarity basal protein complex in epithelial-derived cells, in human and mouse RPE cells in both control and pathological conditions. We found that Scribble specifically localizes at the basolateral membrane of mouse and human RPE cells. In addition, we observed an increase in the expression of Scribble during human RPE development in culture, while it acquires a well-defined basolateral pattern as this process is completed. Finally, the expression and location of Scribble were analyzed in h

SUBMITTER: Segurado A 

PROVIDER: S-EPMC9539273 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature