Generation of a host cell line containing a MAR-rich landing pad for site-specific integration and expression of transgenes.

ABSTRACT: In recent years, targeted gene integration (TI) has been introduced as a strategy for the generation of recombinant mammalian cell lines for the production of biotherapeutics. Besides reducing the immense heterogeneity within a pool of recombinant transfectants, TI also aims at shortening the duration of the current cell line development process. Here we describe the generation of a host cell line carrying Matrix-Attachment Region (MAR)-rich landing pads (LPs), which allow for the simultaneous and site-specific integration of multiple genes of interest (GOIs). We show that several copies of each chicken lysozyme 5'MAR-based LP containing either BxB1 wild type or mutated recombination sites, integrated at one random chromosomal locus of the host cell genome. We further demonstrate that thes

SUBMITTER: Oliviero C 

PROVIDER: S-EPMC9539524 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature