Magnesium(II)-ATP Complexes in 1-Ethyl-3-Methylimidazolium Acetate Solutions Characterized by ³¹ Mg β-Radiation-Detected NMR Spectroscopy.

ABSTRACT: The complexation of Mg^II with adenosine 5'-triphosphate (ATP) is omnipresent in biochemical energy conversion, but is difficult to interrogate directly. Here we use the spin- 1/2 β-emitter ³¹ Mg to study Mg^II -ATP complexation in 1-ethyl-3-methylimidazolium acetate (EMIM-Ac) solutions using β-radiation-detected nuclear magnetic resonance (β-NMR). We demonstrate that (nuclear) spin-polarized ³¹ Mg, following ion-implantation from an accelerator beamline into EMIM-Ac, binds to ATP within its radioactive lifetime before depolarizing. The evolution of the spectra with solute concentration indicates that the implanted ³¹ Mg initially bind to the solvent acetate anions, whereafter they undergo dynamic exchange and form either a mono- (³

SUBMITTER: McFadden RML 

PROVIDER: S-EPMC9539566 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature