Regulation of heart rate following genetic deletion of the ß1 adrenergic receptor in larval zebrafish.

ABSTRACT:

Aim

Although zebrafish are gaining popularity as biomedical models of cardiovascular disease, our understanding of their cardiac control mechanisms is fragmentary. Our goal was to clarify the controversial role of the ß1-adrenergic receptor (AR) in the regulation of heart rate in zebrafish.

Methods

CRISPR-Cas9 was used to delete the adrb1 gene in zebrafish allowing us to generate a stable adrb1^-/- line. Larval heart rates were measured during pharmacological protocols and with exposure to hypercapnia. Expression of the five zebrafish adrb genes were measured in larval zebrafish hearts using qPCR.

Results

Compared with genetically matched wild-types (adrb1^+/+ ), adrb1^-/- larvae exhibited ~20 beats min^-1 lower heart rate, meas

SUBMITTER: Joyce W 

PROVIDER: S-EPMC9539580 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature