Modification of Diet to Reduce the Stemness and Tumorigenicity of Murine and Human Intestinal Cells.

ABSTRACT:

Scope

Black raspberries (BRBs) have colorectal cancer (CRC) chemo-preventative effects. As CRC originates from an intestinal stem cell (ISC) this study has investigated the impact of BRBs on normal and mutant ISCs.

Methods and results

Mice with an inducible Apc^fl mutation in either the ISC (Lgr5CreER^T2 ) or intestinal crypt (AhCre/VillinCreER^T2 ) are fed a control or 10% BRB-supplemented diet. This study uses immunohistochemistry, gene expression analysis, and organoid culture to evaluate the effect of BRBs on intestinal homeostasis. RNAscope is performed for ISC markers on CRC adjacent normal colonic tissue pre and post BRB intervention from patients. 10% BRB diet has no overt effect on murine intestinal homeostasis, despite a reduced stem c

SUBMITTER: May S 

PROVIDER: S-EPMC9539894 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature