The in vivo Interaction Landscape of Histones H3.1 and H3.3.

ABSTRACT: Chromatin structure, transcription, DNA replication, and repair are regulated via locus-specific incorporation of histone variants and posttranslational modifications that guide effector chromatin-binding proteins. Here we report unbiased, quantitative interactomes for the replication-coupled (H3.1) and replication-independent (H3.3) histone H3 variants based on BioID proximity labeling, which allows interactions in intact, living cells to be detected. Along with a significant proportion of previously reported interactions detected by affinity purification followed by mass spectrometry, three quarters of the 608 histone-associated proteins that we identified are new, uncharacterized histone associations. The data reveal important biological nuances not captured by traditional biochemical m

SUBMITTER: Siddaway R 

PROVIDER: S-EPMC9540345 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature