Divide and Conquer: A Tailored Solid-state NMR Approach to Study Large Membrane Protein Complexes.

ABSTRACT: Membrane proteins are known to exert many essential biological functions by forming complexes in cell membranes. An example refers to the β-barrel assembly machinery (BAM), a 200 kDa pentameric complex containing BAM proteins A-E that catalyzes the essential process of protein insertion into the outer membrane of gram-negative bacteria. While progress has been made in capturing three-dimensional structural snapshots of the BAM complex, the role of the lipoprotein BamC in the complex assembly in functional lipid bilayers has remained unclear. We have devised a component-selective preparation scheme to directly study BamC as part of the entire BAM complex in lipid bilayers. Combination with proton-detected solid-state NMR methods allowed us to probe the structure, dynamics, and supramolecula

SUBMITTER: Xiang S 

PROVIDER: S-EPMC9540533 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature