Expression of membrane fusion proteins in spermatozoa and total fertilisation failure during in vitro fertilisation.

ABSTRACT:

Background

Couples increasingly experience infertility and seek help from assisted reproductive techniques to become pregnant. However, 5%-15% of the couples that are selected for in vitro fertilisation (IVF) experience a total fertilisation failure (TFF), where no zygotes develop despite oocytes and semen parameters appear to be normal. We hypothesise that TFF during IVF could be related to improper membrane fusion of gametes.

Objective

To investigate the membrane integrity and fusion proteins in spermatozoa from men in couples experiencing TFF.

Materials and methods

A total of 33 infertile couples, 17 of which experienced TFF during IVF and 16 matched control couples with normal IVF fertilisation rates, were selected and the men re-called to deliver an additional se

SUBMITTER: Enoiu SI 

PROVIDER: S-EPMC9540887 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature