Ca_v 3.2 T-type calcium channel regulates mouse platelet activation and arterial thrombosis.

ABSTRACT:

Background

Ca_v 3.2 is a T-type calcium channel that causes low-threshold exocytosis. T-type calcium channel blockers reduce platelet granule exocytosis and aggregation. However, studies of the T-type calcium channel in platelets are lacking.

Objective

To examine the expression and role of Ca_v 3.2 in platelet function.

Methods

Global Ca_v 3.2^-/- and platelet-specific Ca_v 3.2^-/- mice and littermate controls were used for this study. Western blot analysis was used to detect the presence of Ca_v 3.2 and activation of the calcium-responsive protein extracellular signal-regulated kinase (ERK). Fura-2 dye was used to assess platelet calcium. Flow cytometry and light transmission aggregometry were used

SUBMITTER: Tamang HK 

PROVIDER: S-EPMC9541131 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature