Exploiting flow cytometry for the unbiased quantification of protein inclusions in Caenorhabditis elegans.

ABSTRACT: The aggregation of proteins into inclusions or plaques is a prominent hallmark of a diverse range of pathologies including neurodegenerative diseases. The quantification of such inclusions in Caenorhabditis elegans models of aggregation is usually achieved by fluorescence microscopy or other techniques involving biochemical fractionation of worm lysates. Here, we describe a simple and rapid flow cytometry-based approach that allows fluorescently tagged inclusions to be enumerated in whole worm lysate in a quantitative and unbiased fashion. We demonstrate that this technique is applicable to multiple C. elegans models of aggregation and importantly, can be used to monitor the dynamics of inclusion formation in response to heat shock and during ageing. This includes the characterisation of p

SUBMITTER: Claesson K 

PROVIDER: S-EPMC9541147 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature