DDA-imaging with structural identification of lipid molecules on an Orbitrap Velos Pro mass spectrometer.

ABSTRACT: Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) is a useful technique for visualizing the spatial distribution of lipid molecules in tissues. Nevertheless, the use of MSI to investigate local lipid metabolic hallmarks has until recently been hampered by a lack of adequate technology that supports confident lipid identification. This limitation was recently mitigated by the development of DDA-imaging technology where high-resolution MSI is combined with parallel acquisition of lipid tandem MS² spectra on a hybrid ion trap-Orbitrap Elite mass spectrometer featuring a resolving power of 240,000 and a scan time of 1 s. Here, we report the key tenets related to successful transfer of the DDA-imaging technology onto an Orbitrap Velos Pro instrument fe

SUBMITTER: Khalil SM 

PROVIDER: S-EPMC9541402 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature