Novel matrix strategies for improved ionization and spatial resolution using IR-MALDESI mass spectrometry imaging.

ABSTRACT: In mass spectrometry imaging (MSI) applications of infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI), an exogenous ice layer is the gold standard for an energy-absorbing matrix. However, the formation of the ice matrix requires additional time and instrument hardware, so glycerol was investigated herein as an alternative to the ice matrix to potentially improve spatial resolution and ionization, while decreasing experiment time. Glycerol solutions of varying concentrations were sprayed over top of rat liver tissue sections for analysis by IR-MALDESI and compared to the typical ice matrix condition. Additionally, we tested if combining the ice matrix and glycerol matrix would further improve analyses. Matrix conditions were evaluated by comparing ion abundance o

SUBMITTER: Kibbe RR 

PROVIDER: S-EPMC9541679 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature