Trunk injection delivery of dsRNA for RNAi-based pest control in apple trees.

ABSTRACT:

Background

RNA interference (RNAi) is a promising new approach for controlling insect pests without the use of synthetic pesticides. Trunk injection is a delivery system for woody plants that harnesses the vascular system of the tree to transport materials to the tree canopy. Full size apple trees were injected with double-stranded RNA (dsRNA), and season-long leaf samples were taken to measure the vascular mobility and temporal persistence of dsRNA, using quantitative reverse transcription polymerase chain reaction (qRT-PCR).

Results

The qRT-PCR results revealed that the quantities of dsRNA in the apple leaves of treated trees were significantly greater than those in the leaves of untreated trees for both 2019 and 2020 studies. The peak dsRNA concentration in 2019 was 242 p

SUBMITTER: Wise JC 

PROVIDER: S-EPMC9541757 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature