Aconitate decarboxylase 1 regulates glucose homeostasis and obesity in mice.

ABSTRACT:

Objective

The intersection between immunology and metabolism contributes to the pathogenesis of obesity-associated metabolic diseases as well as molecular control of inflammatory responses. The metabolite itaconate and the cell-permeable derivatives have robust anti-inflammatory effects; therefore, it is hypothesized that cis-aconitate decarboxylase (Acod1)-produced itaconate has a protective, anti-inflammatory effect during diet-induced obesity and metabolic disease.

Methods

Wild-type and Acod1^-/- mice were subjected to diet-induced obesity. Glucose metabolism was analyzed by glucose tolerance tests, insulin tolerance tests, and indirect calorimetry. Gene expression and transcriptome analysis was performed using quantitative reverse transcription-polymerase chain

SUBMITTER: Frieler RA 

PROVIDER: S-EPMC9541899 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature