Semi-automated background removal limits data loss and normalizes imaging mass cytometry data.

ABSTRACT: Imaging mass cytometry (IMC) allows the detection of multiple antigens (approximately 40 markers) combined with spatial information, making it a unique tool for the evaluation of complex biological systems. Due to its widespread availability and retained tissue morphology, formalin-fixed, paraffin-embedded (FFPE) tissues are often a material of choice for IMC studies. However, antibody performance and signal to noise ratios can differ considerably between FFPE tissues as a consequence of variations in tissue processing, including fixation. In contrast to batch effects caused by differences in the immunodetection procedure, variations in tissue processing are difficult to control. We investigated the effect of immunodetection-related signal intensity fluctuations on IMC analysis and phenoty

SUBMITTER: Ijsselsteijn ME 

PROVIDER: S-EPMC9542015 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature