A commercial SnF₂ toothpaste formulation reduces simulated human plaque biofilm in a dynamic typodont model.

ABSTRACT:

Aims

We present a dynamic typodont biofilm model (DTBM) incorporating (1) human dentition anatomy, (2) fluid flow over intermittently fluid bathed tooth surfaces and (3) an oxic headspace to allow aerobic and anaerobic niches to develop naturally, as a screening tool to assess the effect of stannous fluoride (SnF₂ ) toothpaste against a simulated human plaque biofilm (SPB).

Methods and results

First, hydroxyapatite (HA) coupons were inoculated with human saliva/plaque and cultured at 37°C under air. Selected species representative of common commensal and anaerobic pathogens were quantified for relative abundance changes over 4 days by PCR densitometry to confirm the culture conditions allowed the proliferation of these species. A continuous culture DTBM reactor on

SUBMITTER: Khosravi Y 

PROVIDER: S-EPMC9542754 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature