CXCL11 expressing C57BL/6 mice have intact adaptive immune responses to viral infection.

ABSTRACT: The chemokine receptor CXCR3 is expressed on immune cells to co-ordinate lymphocyte activation and migration. CXCR3 binds three chemokine ligands, CXCL9, CXCL10 and CXCL11. These ligands display distinct expression patterns and ligand signaling biases; however, how each ligand functions individually and collaboratively is incompletely understood. CXCL9 and CXCL10 are considered pro-inflammatory chemokines during viral infection, while CXCL11 may induce a tolerizing state. The investigation of the individual role of CXCL11 in vivo has been hampered as C57BL/6 mice carry several mutations that result in a null allele. Here, CRISPR/Cas9 was used to correct these mutations on a C57BL/6 background. It was validated that CXCL11^KI mice expressed CXCL11 protein in dendritic cells, splee

SUBMITTER: Dalit L 

PROVIDER: S-EPMC9542850 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature