ICAP-1 loss impairs CD8⁺ thymocyte development and leads to reduced marginal zone B cells in mice.

ABSTRACT: ICAP-1 regulates β1-integrin activation and cell adhesion. Here, we used ICAP-1-null mice to study ICAP-1 potential involvement during immune cell development and function. Integrin α4β1-dependent adhesion was comparable between ICAP-1-null and control thymocytes, but lack of ICAP-1 caused a defective single-positive (SP) CD8⁺ cell generation, thus, unveiling an ICAP-1 involvement in SP thymocyte development. ICAP-1 bears a nuclear localization signal and we found it displayed a strong nuclear distribution in thymocytes. Interestingly, there was a direct correlation between the lack of ICAP-1 and reduced levels in SP CD8⁺ thymocytes of Runx3, a transcription factor required for CD8⁺ thymocyte generation. In the spleen, ICAP-1 was found evenly distributed be

SUBMITTER: Sevilla-Movilla S 

PROVIDER: S-EPMC9543158 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature