Mass spectrometry-based tear proteomics for noninvasive biomarker discovery.

ABSTRACT: The lacrimal film has attracted increasing interest in the last decades as a potential source of biomarkers of physiopathological states, due to its accessibility, moderate complexity, and responsiveness to ocular and systemic diseases. High-performance liquid chromatography-mass spectrometry (LC-MS) has led to effective approaches to tear proteomics, despite the intrinsic limitations in sample amounts. This review focuses on the recent progress in strategy and technology, with an emphasis on the potential for personalized medicine. After an introduction on lacrimal-film composition, examples of applications to biomarker discovery are discussed, comparing approaches based on pooled-sample and single-tear analysis. Then, the most critical steps of the experimental pipeline, that is, tear co

SUBMITTER: Ponzini E 

PROVIDER: S-EPMC9543345 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature