Exact mass GC-MS analysis: Protocol, database, advantages and application to plant metabolic profiling.

ABSTRACT: Plant metabolomics has been used widely in plant physiology, in particular to analyse metabolic responses to environmental parameters. Derivatization (via trimethylsilylation and methoximation) followed by GC-MS metabolic profiling is a major technique to quantify low molecular weight, common metabolites of primary carbon, sulphur and nitrogen metabolism. There are now excellent opportunities for new generation analyses, using high resolution, exact mass GC-MS spectrometers that are progressively becoming relatively cheap. However, exact mass GC-MS analyses for routine metabolic profiling are not common, since there is no dedicated available database. Also, exact mass GC-MS is usually dedicated to structural resolution of targeted secondary metabolites. Here, we present a curated database

SUBMITTER: Abadie C 

PROVIDER: S-EPMC9543805 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature