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Crystal Structure Based Mutagenesis of Cattleyene Synthase Leads to the Generation of Rearranged Polycyclic Diterpenes.

ABSTRACT: The crystal structures of cattleyene synthase (apo-CyS), and CyS complexed with geranylgeranyl pyrophosphate (GGPP) were solved. The CyS^C59A variant exhibited an increased production of cattleyene and other diterpenes with diverse skeletons. Its structure showed a widened active site cavity explaining the relaxed selectivity. Isotopic labeling experiments revealed a remarkable cyclization mechanism involving several skeletal rearrangements for one of the novel diterpenes.

SUBMITTER: Xing B

PROVIDER: S-EPMC9543850 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature

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Crystal Structure Based Mutagenesis of Cattleyene Synthase Leads to the Generation of Rearranged Polycyclic Diterpenes.

Xing Baiying B Xu Houchao H Li Annan A Lou Tingting T Xu Meng M Wang Kaibiao K Xu Zhengren Z Dickschat Jeroen S JS Yang Donghui D Ma Ming M

Angewandte Chemie (International ed. in English) 20220801 36

The crystal structures of cattleyene synthase (apo-CyS), and CyS complexed with geranylgeranyl pyrophosphate (GGPP) were solved. The CyS<sup>C59A</sup> variant exhibited an increased production of cattleyene and other diterpenes with diverse skeletons. Its structure showed a widened active site cavity explaining the relaxed selectivity. Isotopic labeling experiments revealed a remarkable cyclization mechanism involving several skeletal rearrangements for one of the novel diterpenes. ...[more]

PMID: 35819825

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