Aptamer-Array-Guided Protein Assembly Enhances Synthetic mRNA Switch Performance.

ABSTRACT: Synthetic messenger RNA (mRNA) switches are powerful synthetic biological tools that can sense cellular molecules to manipulate cell fate. However, their performances are limited by high output signal noise due to leaky output protein expression. Here, we designed a readout control module that disables protein leakage from generating signal. Aptamer array on the switch guides the inactive output protein to self-assemble into functional assemblies that generate output signal. Leaky protein expression fails to saturate the array, thus produces marginal signal. In this study, we demonstrated that switches with this module exhibit substantially lower signal noise and, consequently, higher input sensitivity and wider output range. Such switches are applicable for different types of input molecu

SUBMITTER: Lu Q 

PROVIDER: S-EPMC9544043 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature