Suppression of Liquid-Liquid Phase Separation and Aggregation of Antibodies by Modest Pressure Application.

ABSTRACT: The high colloidal stability of antibody (immunoglobulin) solutions is important for pharmaceutical applications. Inert cosolutes, excipients, are generally used in therapeutic protein formulations to minimize physical instabilities, such as liquid-liquid phase separation (LLPS), aggregation and precipitation, which are often encountered during manufacturing and storage. Despite their widespread use, a detailed understanding of how excipients modulate the specific protein-protein interactions responsible for these instabilities is still lacking. In this work, we demonstrate the high sensitivity to pressure of globulin condensates as a suitable means to suppress LLPS and subsequent aggregation of concentrated antibody solutions. The addition of excipients has only a minor effect. The high p

SUBMITTER: Fetahaj Z 

PROVIDER: S-EPMC9544093 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature