Regulation of transcription of the Bacillus subtilis pyrG gene, encoding cytidine triphosphate synthetase.

ABSTRACT: The B. subtilis pyrG gene, which encodes CTP synthetase, is located far from the pyrimidine biosynthetic operon on the chromosome and is independently regulated. The pyrG promoter and 5' leader were fused to lacZ and integrated into the chromosomes of several B. subtilis strains having mutations in genes of pyrimidine biosynthesis and salvage. These mutations allowed the intracellular pools of cytidine and uridine nucleotides to be manipulated by the composition of the growth medium. These experiments indicated that pyrG expression is repressed by cytidine nucleotides but is largely independent of uridine nucleotides. The start of pyrG transcription was mapped by primer extension to a position 178 nucleotides upstream of the translation initiation codon. A factor-independent termination ha

SUBMITTER: Meng Q 

PROVIDER: S-EPMC95441 | biostudies-literature | 2001 Oct

REPOSITORIES: biostudies-literature