Augmenting glycosylation-directed folding pathways enhances the fidelity of HIV Env immunogen production in plants.

ABSTRACT: Heterologous glycoprotein production relies on host glycosylation-dependent folding. When the biosynthetic machinery differs from the usual expression host, there is scope to remodel the assembly pathway to enhance glycoprotein production. Here we explore the integration of chaperone coexpression with glyco-engineering to improve the production of a model HIV-1 envelope antigen. Calreticulin was coexpressed to support protein folding together with Leishmania major STT3D oligosaccharyltransferase, to improve glycan occupancy, RNA interference to suppress the formation of truncated glycans, and Nicotiana benthamiana plants lacking α1,3-fucosyltransferase and β1,2-xylosyltransferase was used as an expression host to prevent plant-specific complex N-glycans forming. This approach reduced the f

SUBMITTER: Margolin E 

PROVIDER: S-EPMC9544252 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature