Pseudomonas aeruginosa modulates neutrophil granule exocytosis in an in vitro model of airway infection.

ABSTRACT: A population of neutrophils recruited into cystic fibrosis (CF) airways is associated with proteolytic lung damage, exhibiting high expression of primary granule exocytosis marker CD63 and reduced phagocytic receptor CD16. Causative factors for this population are unknown, limiting intervention. Here we present a laboratory model to characterize responses of differentiated airway epithelium and neutrophils following respiratory infection. Pediatric primary airway epithelial cells were cultured at the air-liquid interface, challenged individually or in combination with rhinovirus (RV) and Pseudomonas aeruginosa, then apically washed with medical saline to sample epithelial infection milieus. Cytokine multiplex analysis revealed epithelial antiviral signals, including IP-10 and RANTES, incre

SUBMITTER: Laucirica DR 

PROVIDER: S-EPMC9544492 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature