Emergence of circulating influenza A H3N2 viruses with genetic drift in the matrix gene: be alert of false-negative test results.
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ABSTRACT:
SUBMITTER: Jorgensen RL
PROVIDER: S-EPMC9544743 | biostudies-literature | 2022 Oct
REPOSITORIES: biostudies-literature
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Emergence of circulating influenza A H3N2 viruses with genetic drift in the matrix gene: be alert of false-negative test results.
Jørgensen Rikke Lind RL Lerche Christian Johann CJ Pedersen Martin Schou MS Kirkby Nikolai Soren NS Botnen Amanda Bolt AB Trebbien Ramona R Nilsson-Møller Stephen S Pinholt Mette M Nielsen Alex Christian Yde ACY Westh Henrik H Lisby Jan Gorm JG Schneider Uffe Vest UV
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 20220810 10
In March 2022, we observed samples with a negative fluorescent signal (60.5%, n = 43) for the influenza A matrix gene and a stronger positive signal for subtype A(H3N2). Forty-three samples were positive in InfA (H3N2) (mean Cq 30.9, range 23.9-35.1), and 26 of the 43 samples were negative in InfA matrix (mean Cq 28.0, range 23.2-30.6). Our multiplex test is a laboratory-developed four-target, four-color influenza A reverse-transcription PCR assay targeting the matrix gene, subtypes A(H3N2) and ...[more]
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