Comparability of Steroid Collision Cross Sections Using Three Different IM-HRMS Technologies: An Interplatform Study.

ABSTRACT: Steroids play key roles in various biological processes and are characterized by many isomeric variants, which makes their unambiguous identification challenging. Ion mobility-mass spectrometry (IM-MS) has been proposed as a suitable platform for this application, particularly using collision cross section (CCS) databases obtained from different commercial IM-MS instruments. CCS is seen as an ideal additional identification parameter for steroids as long-term repeatability and interlaboratory reproducibility of this measurand are excellent and matrix effects are negligible. While excellent results were demonstrated for individual IM-MS technologies, a systematic comparison of CCS derived from all major commercial IM-MS technologies has not been performed. To address th

SUBMITTER: Feuerstein ML 

PROVIDER: S-EPMC9545150 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature