Activation of succinate receptor 1 boosts human mast cell reactivity and allergic bronchoconstriction.

ABSTRACT:

Background

SUCNR1 is a sensor of extracellular succinate, a Krebs cycle intermediate generated in excess during oxidative stress and has been linked to metabolic regulation and inflammation. While mast cells express SUCNR1, its role in mast cell reactivity and allergic conditions such as asthma remains to be elucidated.

Methods

Cord blood-derived mast cells and human mast cell line LAD-2 challenged by SUCNR1 ligands were analyzed for the activation and mediator release. Effects on mast cell-dependent bronchoconstriction were assessed in guinea pig trachea and isolated human small bronchi challenged with antigen and anti-IgE, respectively.

Results

SUCNR1 is abundantly expressed on human mast cells. Challenge with succinate, or the synthetic non-metabolite agonist cis-e

SUBMITTER: Tang X 

PROVIDER: S-EPMC9545225 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature