Dataset Information

The diagnostic accuracy of Sysmex XN for identification of pseudothrombocytopenia using various thresholds for definition of platelet aggregation.

ABSTRACT:

Introduction

The aim of this study was to investigate the diagnostic accuracy of the flag PLT-Clumps from the WNR/WDF and the PLT-F channels from Sysmex XN and to study how different cut-offs for investigation for pseudothrombocytopenia (PTCP) and the definition of platelet aggregation affected the diagnostic accuracy.

Methods

A smear review was performed for samples with platelet count <150 × 10⁹ /L and samples flagged for platelet aggregation by Sysmex XN-20. The samples were investigated for platelet aggregation in 30 fields using 40× objective. Findings were classified by size and quantity using two definitions of aggregation: the Norwegian quality improvement of laboratory investigations (Noklus) and the Groupe Francophone de'Hematologie Cellulaire (GFHC). The Q-values for the PLT-Clumps flag from the WNR/WFD channel and the PLT-F channel were compared with smear findings.

Results

ROC analysis showed that the diagnostic accuracy of the PLT-clumps flags increased with increasing stringency of the definition of platelet aggregation and when only samples with thrombocytopenia were investigated. With the most stringent PTCP definitions, the diagnostic accuracy of the PLT-Clumps flag from PLT-F was very high (AUC 0.97-0.98) and markedly better than for WNR/WDF.

Conclusion

The diagnostic accuracy of PLT-F from Sysmex XN-20 for identification of PTCP was very good and superior to the WNDR/WDF channel in samples with platelet count <150 × 10⁹ /L and a moderate to high number of aggregates in the smear. There is a need for a more precise definition of platelet aggregation.

SUBMITTER: Lunde HE

PROVIDER: S-EPMC9545299 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature

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Publications

The diagnostic accuracy of Sysmex XN for identification of pseudothrombocytopenia using various thresholds for definition of platelet aggregation.

Lunde Hanne Elisabeth HE Hjelmtvedt Agnete Nyborg AN Amundsen Erik Koldberg EK

International journal of laboratory hematology 20220624 5

<h4>Introduction</h4>The aim of this study was to investigate the diagnostic accuracy of the flag PLT-Clumps from the WNR/WDF and the PLT-F channels from Sysmex XN and to study how different cut-offs for investigation for pseudothrombocytopenia (PTCP) and the definition of platelet aggregation affected the diagnostic accuracy.<h4>Methods</h4>A smear review was performed for samples with platelet count <150 × 10<sup>9</sup> /L and samples flagged for platelet aggregation by Sysmex XN-20. The samp ...[more]

PMID: 35751410

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Project description:Background Cell population data (CPD) parameters related to neutrophils, such as fluorescent light intensity (NE-SFL) and fluorescent light distribution width index (NE-WY), have emerged as potential biomarkers for sepsis. However, the diagnostic implication in acute bacterial infection remains unclear. This study assessed the diagnostic value of NE-WY and NE-SFL for bacteremia in patients with acute bacterial infections, and those associations with other sepsis biomarkers. Methods Patients with acute bacterial infections were enrolled in this prospective observational cohort study. For all patients, a blood sample, with at least two sets of blood cultures, were collected at the onset of infection. Microbiological evaluation included examination of the blood bacterial load using PCR. CPD was assessed using Automated Hematology analyzer Sysmex series XN-2000. Serum levels of procalcitonin (PCT), interleukin-6 (IL-6), presepsin, and CRP were also assessed. Results Of 93 patients with acute bacterial infection, 24 developed culture-proven bacteremia and 69 did not. NE-SFL and NE-WY were significantly higher in patients with bacteremia than in those without bacteremia (p < 0.005, respectively), and were significantly correlated with the bacterial load determined by PCR (r = 0.384 and r = 0.374, p < 0.005, respectively). To assess the diagnostic value for bacteremia, receiver operating characteristic curve analysis was used. NE-SFL and NE-WY showed an area under the curve of 0.685 and 0.708, respectively, while those of PCT, IL-6, presepsin, and CRP were 0.744, 0.778, 0.685, and 0.528, respectively. Correlation analysis showed that the levels of NE-WY and NE-SFL were strongly correlated with PCT and IL-6 levels. Conclusion This study demonstrated that NE-WY and NE-SFL could predict bacteremia in a manner that may be different from that of other indicators. These findings suggest there are potential benefits of NE-WY/NE-SFL in predicting severe bacterial infections.

Dataset Information

The diagnostic accuracy of Sysmex XN for identification of pseudothrombocytopenia using various thresholds for definition of platelet aggregation.

Introduction

Methods

Results

Conclusion

Publications

The diagnostic accuracy of Sysmex XN for identification of pseudothrombocytopenia using various thresholds for definition of platelet aggregation.

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