Fitness effects of CRISPR endonucleases in Drosophila melanogaster populations.

ABSTRACT: Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 provides a highly efficient and flexible genome editing technology with numerous potential applications ranging from gene therapy to population control. Some proposed applications involve the integration of CRISPR/Cas9 endonucleases into an organism's genome, which raises questions about potentially harmful effects to the transgenic individuals. One example for which this is particularly relevant are CRISPR-based gene drives conceived for the genetic alteration of entire populations. The performance of such drives can strongly depend on fitness costs experienced by drive carriers, yet relatively little is known about the magnitude and causes of these costs. Here, we assess the fitness effects of genomic CRISPR/Cas9 exp

SUBMITTER: Langmuller AM 

PROVIDER: S-EPMC9545523 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature