Calibrating Catalytic DNA Nanostructures for Site-Selective Protein Modification.

ABSTRACT: Many biomedical fields rely on proteins that are selectively modified. These can be attached using reactive or catalytic moieties, but the position where these moieties are attached is often poorly controlled. We assessed how catalyst position affects the efficiency and selectivity of protein modification. For this, we anchored a template DNA strand to three different proteins, which were subsequently hybridized to DNA strands that contained catalysts at different positions. We found a strong correlation between the catalyst-to-protein distance and the efficiency of protein modification for acyl transfer catalysts, which operate via a covalently bound reactant intermediate. Additionally, we found that the catalyst's distance and orientation with respect to the protein surface, also influen

SUBMITTER: Keijzer JF 

PROVIDER: S-EPMC9546015 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature