BaRTv2: a highly resolved barley reference transcriptome for accurate transcript-specific RNA-seq quantification.

ABSTRACT: Accurate characterisation of splice junctions (SJs) as well as transcription start and end sites in reference transcriptomes allows precise quantification of transcripts from RNA-seq data, and enables detailed investigations of transcriptional and post-transcriptional regulation. Using novel computational methods and a combination of PacBio Iso-seq and Illumina short-read sequences from 20 diverse tissues and conditions, we generated a comprehensive and highly resolved barley reference transcript dataset from the European 2-row spring barley cultivar Barke (BaRTv2.18). Stringent and thorough filtering was carried out to maintain the quality and accuracy of the SJs and transcript start and end sites. BaRTv2.18 shows increased transcript diversity and completeness compared with an earlier ve

SUBMITTER: Coulter M 

PROVIDER: S-EPMC9546494 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature