Dissection of the long-range projections of specific neurons at the synaptic level in the whole mouse brain.

ABSTRACT: Through synaptic connections, long-range circuits transmit information among neurons and connect different brain regions to form functional motifs and execute specific functions. Tracing the synaptic distribution of specific neurons requires submicron-level resolution information. However, it is a great challenge to map the synaptic terminals completely because these fine structures span multiple regions, even in the whole brain. Here, we develop a pipeline including viral tracing, sample embedding, fluorescent micro-optical sectional tomography, and big data processing. We mapped the whole-brain distribution and architecture of long projections of the parvalbumin neurons in the basal forebrain at the synaptic level. These neurons send massive projections to multiple downstream regions wit

SUBMITTER: Tian J 

PROVIDER: S-EPMC9546530 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature