Label-free single-particle imaging approach for ultra-rapid detection of pathogenic bacteria in clinical samples.

ABSTRACT: Rapid detection of pathogenic bacteria within a few minutes is the key to control infectious disease. However, rapid detection of pathogenic bacteria in clinical samples is quite a challenging task due to the complex matrix, as well as the low abundance of bacteria in real samples. Herein, we employ a label-free single-particle imaging approach to address this challenge. By tracking the scattering intensity variation of single particles in free solution, the morphological heterogeneity can be well identified with particle size smaller than the diffraction limit, facilitating the morphological identification of single bacteria from a complex matrix in a label-free manner. Furthermore, the manipulation of convection in free solution enables the rapid screening of low-abundance bacteria in a

SUBMITTER: Chen S 

PROVIDER: S-EPMC9546574 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature