Highly sensitive single-cell chromatin accessibility assay and transcriptome coassay with METATAC.

ABSTRACT: Recent advances in single-cell assay for transposase accessible chromatin using sequencing (scATAC-seq) and its coassays have transformed the field of single-cell epigenomics and transcriptomics. However, the low detection efficiency of current methods has limited our understanding of the true complexity of chromatin accessibility and its relationship with gene expression in single cells. Here, we report a high-sensitivity scATAC-seq method, termed multiplexed end-tagging amplification of transposase accessible chromatin (METATAC), which detects a large number of accessible sites per cell and is compatible with automation. Our high detectability and statistical framework allowed precise linking of enhancers to promoters without merging single cells. We systematically investigated allele-sp

SUBMITTER: Wu H 

PROVIDER: S-EPMC9546615 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature