Novel biochemical, structural, and systems insights into inflammatory signaling revealed by contextual interaction proteomics.

ABSTRACT: Protein-protein interactions (PPIs) represent the main mode of the proteome organization in the cell. In the last decade, several large-scale representations of PPI networks have captured generic aspects of the functional organization of network components but mostly lack the context of cellular states. However, the generation of context-dependent PPI networks is essential for structural and systems-level modeling of biological processes-a goal that remains an unsolved challenge. Here we describe an experimental/computational strategy to achieve a modeling of PPIs that considers contextual information. This strategy defines the composition, stoichiometry, temporal organization, and cellular requirements for the formation of target assemblies. We used this approach to generate an integrated

SUBMITTER: Ciuffa R 

PROVIDER: S-EPMC9546619 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature