Discovering design principles of collagen molecular stability using a genetic algorithm, deep learning, and experimental validation.

ABSTRACT: Collagen is the most abundant structural protein in humans, providing crucial mechanical properties, including high strength and toughness, in tissues. Collagen-based biomaterials are, therefore, used for tissue repair and regeneration. Utilizing collagen effectively during materials processing ex vivo and subsequent function in vivo requires stability over wide temperature ranges to avoid denaturation and loss of structure, measured as melting temperature (T_m). Although significant research has been conducted on understanding how collagen primary amino acid sequences correspond to T_m values, a robust framework to facilitate the design of collagen sequences with specific T_m remains a challenge. Here, we develop a general model using a genetic algorithm with

SUBMITTER: Khare E 

PROVIDER: S-EPMC9546622 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature