Molecular Characterization of Animal Fasciola Spp. Isolates from Lorestan Province, Western Iran.

ABSTRACT:

Background

We aimed to detect the genetic diversity of samples identified morphologically as Fasciola spp. from sheep, cattle and goat from Lorestan Province, western Iran using PCR-RFLP method. Besides, we evaluated the genetic diversity indices, sequencing and phylogenetic analysis using mitochondrial gene (ND1 and CO1).

Methods

PCR-RFLP analysis of ribosomal ITS1 fragment by RsaI restriction enzyme to investigate the genetic characteristics of Fasciola species obtained from different hosts (18 sheep, 21 cattle, and 17goats) was conducted. The samples were sequenced. Sequences were evaluated using BLAST software and the parasite species were identified with similarity percentage and overlap with the species registered in the gene b

SUBMITTER: Heydarian P 

PROVIDER: S-EPMC9546809 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature