Development of intensiometric indicators for visualizing N-cadherin interaction across cells.

ABSTRACT: N-cadherin (NCad) is a classical cadherin that mediates cell-cell interactions in a Ca²⁺-dependent manner. NCad participates in various biological processes, from ontogenesis to higher brain functions, though the visualization of NCad interactions in living cells remains limited. Here, we present intensiometric NCad interaction indicators, named INCIDERs, that utilize dimerization-dependent fluorescent proteins. INCIDERs successfully visualize reversible NCad interactions across cells. Compared to FRET-based indicators, INCIDERs have a ~70-fold higher signal contrast, enabling clear identification of NCad interactions. In primary neuronal cells, NCad interactions are visualized between closely apposed processes. Furthermore, visualization of NCad interaction at cell adhesion sit

SUBMITTER: Kanadome T 

PROVIDER: S-EPMC9546846 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature