Betaine promotes osteogenic differentiation in immortalized human dental pulp-derived cells.

ABSTRACT:

Objectives

This study aimed to evaluate the effect of betaine (BET) on immortalized human dental pulp stem cell (ihDP) osteogenic differentiation.

Materials and methods

hDPs were immortalized using SV40 T-antigen transfection. Characterization, multilineage differentiation, proliferation, cell cycle, colony-forming unit, and cellular senescence were evaluated (n = 4). The effect of BET on ihDP response was assessed (n = 4). Osteogenic differentiation was detected using ALP, ARS staining, and RT-qPCR (n = 4). To investigate the involvement of calcium signaling, the cells were pretreated with either 8-(NN-diethylamino)octyl-3,4,5-trimethoxybenzoate (TMB-8) or thapsigargin before BET treatment (n = 6).

Results

ihDPs retained similar phenotypic characteristics presented i

SUBMITTER: Kornsuthisopon C 

PROVIDER: S-EPMC9546879 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature