A multi-omics longitudinal study of the murine retinal response to chronic low-dose irradiation and simulated microgravity.

ABSTRACT: The space environment includes unique hazards like radiation and microgravity which can adversely affect biological systems. We assessed a multi-omics NASA GeneLab dataset where mice were hindlimb unloaded and/or gamma irradiated for 21 days followed by retinal analysis at 7 days, 1 month or 4 months post-exposure. We compared time-matched epigenomic and transcriptomic retinal profiles resulting in a total of 4178 differentially methylated loci or regions, and 457 differentially expressed genes. Highest correlation in methylation difference was seen across different conditions at the same time point. Nucleotide metabolism biological processes were enriched in all groups with activation at 1 month and suppression at 7 days and 4 months. Genes and processes related to Notch and Wnt signaling

SUBMITTER: Kothiyal P 

PROVIDER: S-EPMC9547011 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature