Dataset Information

Dynamic analysis of Tapping Panel Dryness in Hevea brasiliensis reveals new insights on this physiological syndrome affecting latex production.

ABSTRACT: Tapping Panel Dryness (TPD) is a physiological disorder affecting natural rubber production in Hevea brasiliensis. TPD is associated with clonal susceptibility and overexploitation of rubber trees. Most studies are based on a binary point view of the absence or presence of TPD. This study sets out to characterize the dynamic of the TPD onset through the monthly monitoring of the dry cut length. This reveals the presence of dry spots on the tapped panel of any trees. The frequency of these dry spots increases dramatically in trees developing high level of TPD. Brown bast is an irreversible form of TPD. Brown bast is correlated to a high level of dry cut length. Application of an intensive harvesting system induces early TPD occurrence, which facilitates the study of TPD. Among latex diagnosis parameters, only sucrose content is significantly associated with TPD. Other parameters are more prone to environmental effects and are not reliable as physiological markers. These findings explain the contradictory conclusions of some papers. This study suggests to use intensive harvesting system and monitor the dry cut length for genetic analysis of TPD.

SUBMITTER: Herlinawati E

PROVIDER: S-EPMC9547236 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature

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Dynamic analysis of Tapping Panel Dryness in <i>Hevea brasiliensis</i> reveals new insights on this physiological syndrome affecting latex production.

Herlinawati Eva E Montoro Pascal P Ismawanto Sigit S Syafaah Afdholiatus A Aji Martini M Giner Michel M Flori Albert A Gohet Eric E Oktavia Fetrina F

Heliyon 20221003 10

Tapping Panel Dryness (TPD) is a physiological disorder affecting natural rubber production in <i>Hevea brasiliensis</i>. TPD is associated with clonal susceptibility and overexploitation of rubber trees. Most studies are based on a binary point view of the absence or presence of TPD. This study sets out to characterize the dynamic of the TPD onset through the monthly monitoring of the dry cut length. This reveals the presence of dry spots on the tapped panel of any trees. The frequency of these ...[more]

PMID: 36217460

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Project description:BACKGROUND: Tapping panel dryness (TPD) is one of the most serious threats to natural rubber production. Although a great deal of effort has been made to study TPD in rubber tree, the molecular mechanisms underlying TPD remain poorly understood. Identification and systematical analyses of the genes associated with TPD are the prerequisites for elucidating the molecular mechanisms involved in TPD. The present study is undertaken to generate information about the genes related to TPD in rubber tree. RESULTS: To identify the genes related to TPD in rubber tree, forward and reverse cDNA libraries from the latex of healthy and TPD trees were constructed using suppression subtractive hybridization (SSH) method. Among the 1106 clones obtained from the two cDNA libraries, 822 clones showed differential expression in two libraries by reverse Northern blot analyses. Sequence analyses indicated that the 822 clones represented 237 unique genes; and most of them have not been reported to be associated with TPD in rubber tree. The expression patterns of 20 differentially expressed genes were further investigated to validate the SSH data by reverse transcription PCR (RT-PCR) and real-time PCR analysis. According to the Gene Ontology convention, 237 unique genes were classified into 10 functional groups, such as stress/defense response, protein metabolism, transcription and post-transcription, rubber biosynthesis, etc. Among the genes with known function, the genes preferentially expressed were associated with stress/defense response in the reverse library, whereas metabolism and energy in the forward one. CONCLUSIONS: The genes associated with TPD were identified by SSH method in this research. Systematic analyses of the genes related to TPD suggest that the production and scavenging of reactive oxygen species (ROS), ubiquitin proteasome pathway, programmed cell death and rubber biosynthesis might play important roles in TPD. Therefore, our results not only enrich information about the genes related to TPD, but also provide new insights into understanding the TPD process in rubber tree.

Dataset Information

Dynamic analysis of Tapping Panel Dryness in Hevea brasiliensis reveals new insights on this physiological syndrome affecting latex production.

Publications

Dynamic analysis of Tapping Panel Dryness in <i>Hevea brasiliensis</i> reveals new insights on this physiological syndrome affecting latex production.

Similar Datasets

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