Dissecting, Fixing, and Visualizing the Drosophila Pupal Notum.

ABSTRACT: The pupae of Drosophila melanogaster are immobile for several days during metamorphosis, during which they develop a new body with a thin transparent adult integument. Their immobility and transparency make them ideal for in vivo live imaging experiments. Many studies have focused on the dorsal epithelial monolayer of the pupal notum because of its accessibility and relatively large size. In addition to the studies of epithelial mechanics and development, the notum has been an ideal tissue to study wound healing. After an injury, the entire epithelial repair process can be captured by live imaging over 6-12 h. Despite the popularity of the notum for live imaging, very few published studies have utilized fixed notum samples. Fixation and staining are common approaches for nearly all other D

SUBMITTER: White JS 

PROVIDER: S-EPMC9547544 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature