Incorporation of an Asymmetric Mo-Fe-S Cluster as an Artificial Cofactor into Nitrogenase.

ABSTRACT: Nitrogenase employs a sophisticated electron transfer system and a Mo-Fe-S-C cofactor, designated the M-cluster [(cit)MoFe₇ S₉ C]), to reduce atmospheric N₂ to bioaccessible NH₃ . Previously, we have shown that the cofactor-free form of nitrogenase can be repurposed as a protein scaffold for the incorporation of a synthetic Fe-S cluster [Fe₆ S₉ (SEt)₂ ]^4- . Here, we demonstrate the utility of an asymmetric Mo-Fe-S cluster [Cp*MoFe₅ S₉ (SH)]^3- as an alternative artificial cofactor upon incorporation into the cofactor-free nitrogenase scaffold. The resultant semi-artificial enzyme catalytically reduces C₂ H₂ to C₂ H₄ , and CN

SUBMITTER: Tanifuji K 

PROVIDER: S-EPMC9547968 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature